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Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories are given and presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles. Subcellular Fractionation contains expert advice and detailed methodology, and will be invaluable to researchers in this important area.

Field flow fractionation (FFF) is an emerging separation technique, which has been proven successful in the analysis of pharmaceuticals, biotechnology products, polymers, soils, and foods, among others. In this book, Martin Schimpf joins forces with Karin Caldwell and J. Calvin Giddings, two of the primary developers of this technique, to bring you the first comprehensive, one-stop reference on the technique.

Field flow fractionation (FFF) is an emerging separation technique, which has been proven successful in the analysis of pharmaceuticals, biotechnology products, polymers, soils, and foods, among others. In this book, Martin Schimpf joins forces with Karin Caldwell and J. Calvin Giddings, two of the primary developers of this technique, to bring you the first comprehensive, one-stop reference on the technique.

"Blood," Goethe observed in Faust, "is a very special juice." How special it is and how complex as well is revealed in Douglas Surgenor's Edwin J. Cohn and the Development of Protein Chemistry. As Surgenor aptly shows, what began as a modest program in basic research at the Harvard Medical School in 1920 with the establishment of a small laboratory for the study of the physical chemistry of proteins, suddenly and quite unexpectedly took on immensely practical proportions twenty years later when the onset of World War II made requisite new sophisticated blood techniques and blood substitutes for the treatment of military casualties. The knowledge and expertise gained by Edwin Cohn and his laboratory associates in the study of proteins, amino acids, and peptides in blood after 1920 put them in a unique position to carry out the search for new blood products. Edwin J. Cohn and the Development of Protein Chemistry discloses how the wartime emergency called into play Cohn's talents as a leader who drew together chemists, clinicians, pathologists, immunologists, and others in the attainment of a complex goal. The revolution Cohn started has still not run its course.

Although modern cell biology is often considered to have arisen following World War II in tandem with certain technological and methodological advances—in particular, the electron microscope and cell fractionation—its origins actually date to the 1830s and the development of cytology, the scientific study of cells. By 1924, with the publication of Edmund Vincent Cowdry’s General Cytology, the discipline had stretched beyond the bounds of purely microscopic observation to include the chemical, physical, and genetic analysis of cells. Inspired by Cowdry’s classic, watershed work, this book collects contributions from cell biologists, historians, and philosophers of science to explore the history and current status of cell biology. Despite extraordinary advances in describing both the structure and function of cells, cell biology tends to be overshadowed by molecular biology, a field that developed contemporaneously. This book remedies that unjust disparity through an investigation of cell biology’s evolution and its role in pushing forward the boundaries of biological understanding. Contributors show that modern concepts of cell organization, mechanistic explanations, epigenetics, molecular thinking, and even computational approaches all can be placed on the continuum of cell studies from cytology to cell biology and beyond. The first book in the series Convening Science: Discovery at the Marine Biological Laboratory, Visions of Cell Biology sheds new light on a century of cellular discovery.

For thousands of years raw colostrum has been consumed to help with all sorts of health challenges. In recent years with the use of modern manufacturing techniques, the benefits of consuming raw colostrum have been preserved in powdered colostrum. In the last ten years more advanced fractionation technologies have allowed the large-scale production of liquid colostrum supplements. Now we are observing a revival in the use of colostrum liquids and powders. Colostrum Peptides, more commonly known as Proline-rich Polypeptides (PRPs) are isolated in these liquid colostrum products. Peptide Immunotherapy is the scientific classification of how these PRPs modulate or balance the human immune system. Constantly balancing and re-teaching the human immune system how to function through passive transfer is the information key to health in all mammals, including humans. So when our immune system faces daily health challenges, we are equipped and ready to defend ourselves down to the molecular levels :.. thanks to Colostrum Peptides.

This Third Edition of the classic, best-selling polymer science textbook surveys theory and practice of all major phases of polymer science, engineering, and technology, including polymerization, solution theory, fractionation and molecular-weight measurement, solid-state properties, structure-property relationships, and the preparation, fabrication and properties of commercially-important plastics, fibers, and elastomers.

Although modern cell biology is often considered to have arisen following World War II in tandem with certain technological and methodological advances—in particular, the electron microscope and cell fractionation—its origins actually date to the 1830s and the development of cytology, the scientific study of cells. By 1924, with the publication of Edmund Vincent Cowdry’s General Cytology, the discipline had stretched beyond the bounds of purely microscopic observation to include the chemical, physical, and genetic analysis of cells. Inspired by Cowdry’s classic, watershed work, this book collects contributions from cell biologists, historians, and philosophers of science to explore the history and current status of cell biology. Despite extraordinary advances in describing both the structure and function of cells, cell biology tends to be overshadowed by molecular biology, a field that developed contemporaneously. This book remedies that unjust disparity through an investigation of cell biology’s evolution and its role in pushing forward the boundaries of biological understanding. Contributors show that modern concepts of cell organization, mechanistic explanations, epigenetics, molecular thinking, and even computational approaches all can be placed on the continuum of cell studies from cytology to cell biology and beyond. The first book in the series Convening Science: Discovery at the Marine Biological Laboratory, Visions of Cell Biology sheds new light on a century of cellular discovery.

A reference text focusing on basic organic chemistry and reactions of naturally occurring organic substances in soils. Covers pools of organic matter in soils, transformations, methods of extraction and fractionation. Section two deals primarily with the chemistry of known classes of organic compounds in soils including saccharides, lipids and constituents containing nitrogen, phosphorus and sulfur. Section three is concerned with basic organic chemistry of humic substances, followed by the importance of organic matter associations and interactions. Contains new chapters on NMR spectroscopy, analytical pyrolysis and on chemical structures.

This is an extensively revised second edition of N. W. Pirie's much acclaimed earlier work, Leaf Protein and Other Aspects of Fodder Fractionation (Cambridge University Press 1978). The fibrous character and flavour of many leaf crops impede their use as green vegetables, and feeding them directly to animals is relatively inefficient. The advantages of separating a fraction for use as human food, and a residue for animal feeding, have gained recognition during the last ten years. In suitable climates, fractionating leafy material can yield more edible protein than other forms of agriculture. The book concludes with a discussion of the problems that arise when leaf protein is made and used in practice, and of the potential role of fodder fractionation in human and animal nutrition. This volume will be of practical importance to students and research workers in animal and human nutrition, agriculture and rural development, particularly those with an interest in developing countries.

A reference text focusing on basic organic chemistry and reactions of naturally occurring organic substances in soils. Covers pools of organic matter in soils, transformations, methods of extraction and fractionation. Section two deals primarily with the chemistry of known classes of organic compounds in soils including saccharides, lipids and constituents containing nitrogen, phosphorus and sulfur. Section three is concerned with basic organic chemistry of humic substances, followed by the importance of organic matter associations and interactions. Contains new chapters on NMR spectroscopy, analytical pyrolysis and on chemical structures.

This classic textbook is an introduction to the systematics and the use of stable isotopes in geosciences. It is subdivided into three parts: i) theoretical and experimental principles, ii) fractionation processes of light and heavy elements, iii) the natural variations of geologically important reservoirs. Since the publication of the previous edition advances in multicollector-ICP-mass-spectrometry allow precise measurements of new isotope systems. In this new edition therefore, 42 elements with resolvable natural variations in isotope composition are discussed. New findings from non-traditional isotope systems have been incorporated. Many new references have been added, which enable quick access to recent literature.

This book presents a quantitative treatment of the theory and natural variations of light stable isotopes. It discusses isotope distribution in the context of fractionation processes, thermodynamics, mass conservation, exchange kinetics, and diffusion theory, and includes more than 100 original equations. The theoretical principles are illustrated with natural examples that emphasize oxygen and hydrogen isotope variations in natural waters, terrestrial and extraterrestrial rocks, and hydrothermal systems. New data on meteoric precipitation, rivers, springs, formation fluids, and hydrothermal systems are included in relation to various natural phenomena. Essentially, this book seeks to reconnect the diverse phenomenological observations of isotope distribution to the quantitative theories of physical chemistry and the language of differential equations. It may serve as a textbook for advanced students, as a research reference, or as a quick source of information. The book is organized into five chapters, each followed by suggested quantitative problems and a short reference list. The three theoretical chapters progress from an elementary review of the physical chemistry of stable isotopes, to the thermodynamics of isotopic compounds, and finally to the calculation of isotope distribution in dynamic systems. The third and fifth chapters emphasize oxygen and hydrogen isotope variations in Earth's hydrosphere and lithosphere, constituting the most important examples of the theoretical principles. Appendices provide data on atomic weights of light elements, physical constants, mathematical relationships, and isotopic fractionation factors.

This book presents a quantitative treatment of the theory and natural variations of light stable isotopes. It discusses isotope distribution in the context of fractionation processes, thermodynamics, mass conservation, exchange kinetics, and diffusion theory, and includes more than 100 original equations. The theoretical principles are illustrated with natural examples that emphasize oxygen and hydrogen isotope variations in natural waters, terrestrial and extraterrestrial rocks, and hydrothermal systems. New data on meteoric precipitation, rivers, springs, formation fluids, and hydrothermal systems are included in relation to various natural phenomena. Essentially, this book seeks to reconnect the diverse phenomenological observations of isotope distribution to the quantitative theories of physical chemistry and the language of differential equations. It may serve as a textbook for advanced students, as a research reference, or as a quick source of information. The book is organized into five chapters, each followed by suggested quantitative problems and a short reference list. The three theoretical chapters progress from an elementary review of the physical chemistry of stable isotopes, to the thermodynamics of isotopic compounds, and finally to the calculation of isotope distribution in dynamic systems. The third and fifth chapters emphasize oxygen and hydrogen isotope variations in Earth's hydrosphere and lithosphere, constituting the most important examples of the theoretical principles. Appendices provide data on atomic weights of light elements, physical constants, mathematical relationships, and isotopic fractionation factors.

This is an extensively revised second edition of N. W. Pirie's much acclaimed earlier work, Leaf Protein and Other Aspects of Fodder Fractionation (Cambridge University Press 1978). The fibrous character and flavour of many leaf crops impede their use as green vegetables, and feeding them directly to animals is relatively inefficient. The advantages of separating a fraction for use as human food, and a residue for animal feeding, have gained recognition during the last ten years. In suitable climates, fractionating leafy material can yield more edible protein than other forms of agriculture. The book concludes with a discussion of the problems that arise when leaf protein is made and used in practice, and of the potential role of fodder fractionation in human and animal nutrition. This volume will be of practical importance to students and research workers in animal and human nutrition, agriculture and rural development, particularly those with an interest in developing countries.

This classic textbook is an introduction to the systematics and the use of stable isotopes in geosciences. It is subdivided into three parts: i) theoretical and experimental principles, ii) fractionation processes of light and heavy elements, iii) the natural variations of geologically important reservoirs. Since the publication of the previous edition advances in multicollector-ICP-mass-spectrometry allow precise measurements of new isotope systems. In this new edition therefore, 42 elements with resolvable natural variations in isotope composition are discussed. New findings from non-traditional isotope systems have been incorporated. Many new references have been added, which enable quick access to recent literature.