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This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.

This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.

Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities. Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitationsProvides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and WatersIncludes biological applications and exploration of analytical tools and databases for bioinformatics

Introduction to Protein Mass Spectrometry provides a comprehensive overview of this increasingly important, yet complex, analytical technique. Unlike many other methods which automatically yield an absolutely unique protein name as output, protein mass spectrometry generally requires a deduction of protein identity from determination of peptide fragmentation products. This book enables readers to both understand, and appreciate, how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. Citing the most recent and relevant work in the field of biological mass spectrometry, the book is written for researchers and scientists new to the field, but is also an ideal resource for those hoping to hone their analytical abilities. Offers introductory information for scientists and researchers new to the field, as well as advanced insight into the critical assessment of computer-analyzed mass spectrometric results and their current limitationsProvides examples of commonly-used MS instruments from Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, and WatersIncludes biological applications and exploration of analytical tools and databases for bioinformatics

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more

Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniquesProvides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniquesProvides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

If you are part of the ''conventional wisdom crowd'', take a very deep breath... with Muscle Explosion you're going to: - Reduce caloric intake to well below maintenance levels and eliminate protein completely (in very specific ways for very specific purposes) - Aim to overtrain - Train the same body part five days in a row - Perform the same exercise five days in a row Muscle Explosion literally turns conventional muscle-building wisdom inside-out and upside down. By practicing the groundbreaking training and eating strategies in this book, you will SHATTER your ''genetic limitations'' by literally changing your physiology, quickly setting the stage for EXPLOSIVE increases in muscle mass and strength. Each cycle of this program lasts only 28 days and the workouts take less than an hour to complete. This book is for the intermediate to advanced trainer who is ready to DEMOLISH plateaus and achieve growth and strength increases previously thought unattainable.

Recent advances in large scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Mass spectrometry is the method of choice for the rapid large-scale identification of these proteomes and their modifications. This is the first book to extensively cover the applications of mass spectrometry to proteome research.

A series of explosions took out the royal motorcade and threw the country of Lydia into chaos. Princess Anastasia missed her limo, and doesn't know what has become of the rest of her family. Whisked away from her native country for her protection, Stasi is dismayed that her life is now in shambles. But at least she's not alone. Kirk Covington pledges to keep her safe. He's a royal guard...and the man she's spent years blaming for her brother's disappearance. Now she'll have to trust him with her life—and her heart—in order to regain the throne.

Proteomics is the study of the subsets of proteins present in different parts of an organism and how they change with time and varying conditions. Mass spectrometry is the leading technology used in proteomics, and the field relies heavily on bioinformatics to process and analyze the acquired data.   Since recent years have seen tremendous developments in instrumentation and proteomics-related bioinformatics, there is clearly a need for a solid introduction to the crossroads where proteomics and bioinformatics meet. Computational Methods for Mass Spectrometry Proteomics describes the different instruments and methodologies used in proteomics in a unified manner. The authors put an emphasis on the computational methods for the different phases of a proteomics analysis, but the underlying principles in protein chemistry and instrument technology are also described. The book is illustrated by a number of figures and examples, and contains exercises for the reader. Written in an accessible yet rigorous style, it is a valuable reference for both informaticians and biologists. Computational Methods for Mass Spectrometry Proteomics is suited for advanced undergraduate and graduate students of bioinformatics and molecular biology with an interest in proteomics. It also provides a good introduction and reference source for researchers new to proteomics, and for people who come into more peripheral contact with the field.

Doctor Szent-Gyorgyi presents his research about the nature of protein and the processes that can underlie both normal and cancerous states.

Proteomics is the study of the subsets of proteins present in different parts of an organism and how they change with time and varying conditions. Mass spectrometry is the leading technology used in proteomics, and the field relies heavily on bioinformatics to process and analyze the acquired data.   Since recent years have seen tremendous developments in instrumentation and proteomics-related bioinformatics, there is clearly a need for a solid introduction to the crossroads where proteomics and bioinformatics meet. Computational Methods for Mass Spectrometry Proteomics describes the different instruments and methodologies used in proteomics in a unified manner. The authors put an emphasis on the computational methods for the different phases of a proteomics analysis, but the underlying principles in protein chemistry and instrument technology are also described. The book is illustrated by a number of figures and examples, and contains exercises for the reader. Written in an accessible yet rigorous style, it is a valuable reference for both informaticians and biologists. Computational Methods for Mass Spectrometry Proteomics is suited for advanced undergraduate and graduate students of bioinformatics and molecular biology with an interest in proteomics. It also provides a good introduction and reference source for researchers new to proteomics, and for people who come into more peripheral contact with the field.

In the second half of the nineteenth century, the Euro-American citizenry of California carried out mass genocide against the Native population of their state, using the processes and mechanisms of democracy to secure land and resources for themselves and their private interests. The murder, rape, and enslavement of thousands of Native people were legitimized by notions of democracy—in this case mob rule—through a discreetly organized and brutally effective series of petitions, referenda, town hall meetings, and votes at every level of California government. Murder State is a comprehensive examination of these events and their early legacy. Preconceptions about Native Americans as shaped by the popular press and by immigrants’ experiences on the Overland Trail to California were used to further justify the elimination of Native people in the newcomers’ quest for land. The allegedly “violent nature” of Native people was often merely their reaction to the atrocities committed against them as they were driven from their ancestral lands and alienated from their traditional resources. In this narrative history employing numerous primary sources and the latest interdisciplinary scholarship on genocide, Brendan C. Lindsay examines the darker side of California history, one rarely studied in detail, and the motives of both Native Americans and Euro-Americans at the time. Murder State calls attention to the misuse of democracy to justify and commit genocide.

In the second half of the nineteenth century, the Euro-American citizenry of California carried out mass genocide against the Native population of their state, using the processes and mechanisms of democracy to secure land and resources for themselves and their private interests. The murder, rape, and enslavement of thousands of Native people were legitimized by notions of democracy—in this case mob rule—through a discreetly organized and brutally effective series of petitions, referenda, town hall meetings, and votes at every level of California government. Murder State is a comprehensive examination of these events and their early legacy. Preconceptions about Native Americans as shaped by the popular press and by immigrants’ experiences on the Overland Trail to California were used to further justify the elimination of Native people in the newcomers’ quest for land. The allegedly “violent nature” of Native people was often merely their reaction to the atrocities committed against them as they were driven from their ancestral lands and alienated from their traditional resources. In this narrative history employing numerous primary sources and the latest interdisciplinary scholarship on genocide, Brendan C. Lindsay examines the darker side of California history, one rarely studied in detail, and the motives of both Native Americans and Euro-Americans at the time. Murder State calls attention to the misuse of democracy to justify and commit genocide.

In 1972, the Bureau of Indian Affairs terminated its twenty-year-old Voluntary Relocation Program, which encouraged the mass migration of roughly 100,000 Native American people from rural to urban areas. At the time the program ended, many groups--from government leaders to Red Power activists--had already classified it as a failure, and scholars have subsequently positioned the program as evidence of America's enduring settler-colonial project. But Douglas K. Miller here argues that a richer story should be told--one that recognizes Indigenous mobility in terms of its benefits and not merely its costs. In their collective refusal to accept marginality and destitution on reservations, Native Americans used the urban relocation program to take greater control of their socioeconomic circumstances. Indigenous migrants also used the financial, educational, and cultural resources they found in cities to feed new expressions of Indigenous sovereignty both off and on the reservation. The dynamic histories of everyday people at the heart of this book shed new light on the adaptability of mobile Native American communities. In the end, this is a story of shared experience across tribal lines, through which Indigenous people incorporated urban life into their ideas for Indigenous futures.

This is the first of three volumes on the history of the Native people of Canada as revealed by archaeological evidence. Over 12,000 years of Native history preceded the arrival of Europeans. This first volume begins with the spread of Ice Age hunters out of a land mass called Beringia that once joined Asia and North America. Most of the country was covered by glacial ice, and extinct animals such as mammoth and sabre toothed cats occupied the tundra and lichen woodlands. People of this first and subsequent migrations from Asia gradually adapted to the rapidly changing environment. Eventually, distinct cultures occupied all of Canada`s major environmental zones. Volumes two and three span the periods from 1,000 B.C. to A.D. 500 and A.D. 500 to European contact, respectively. It is hoped that these volumes will make a contribution towards a greater appreciation of Native history prior to the devastating events initiated by the European occupation of Canada.

This is the first of three volumes on the history of the Native people of Canada as revealed by archaeological evidence. Over 12,000 years of Native history preceded the arrival of Europeans. This first volume begins with the spread of Ice Age hunters out of a land mass called Beringia that once joined Asia and North America. Most of the country was covered by glacial ice, and extinct animals such as mammoth and sabre toothed cats occupied the tundra and lichen woodlands. People of this first and subsequent migrations from Asia gradually adapted to the rapidly changing environment. Eventually, distinct cultures occupied all of Canada`s major environmental zones. Volumes two and three span the periods from 1,000 B.C. to A.D. 500 and A.D. 500 to European contact, respectively. It is hoped that these volumes will make a contribution towards a greater appreciation of Native history prior to the devastating events initiated by the European occupation of Canada.

In the second half of the nineteenth century, the Euro-American citizenry of California carried out mass genocide against the Native population of their state, using the processes and mechanisms of democracy to secure land and resources for themselves and their private interests. The murder, rape, and enslavement of thousands of Native people were legitimized by notions of democracy—in this case mob rule—through a discreetly organized and brutally effective series of petitions, referenda, town hall meetings, and votes at every level of California government. Murder State is a comprehensive examination of these events and their early legacy. Preconceptions about Native Americans as shaped by the popular press and by immigrants’ experiences on the Overland Trail to California were used to further justify the elimination of Native people in the newcomers’ quest for land. The allegedly “violent nature” of Native people was often merely their reaction to the atrocities committed against them as they were driven from their ancestral lands and alienated from their traditional resources. In this narrative history employing numerous primary sources and the latest interdisciplinary scholarship on genocide, Brendan C. Lindsay examines the darker side of California history, one rarely studied in detail, and the motives of both Native Americans and Euro-Americans at the time. Murder State calls attention to the misuse of democracy to justify and commit genocide.

In the second half of the nineteenth century, the Euro-American citizenry of California carried out mass genocide against the Native population of their state, using the processes and mechanisms of democracy to secure land and resources for themselves and their private interests. The murder, rape, and enslavement of thousands of Native people were legitimized by notions of democracy—in this case mob rule—through a discreetly organized and brutally effective series of petitions, referenda, town hall meetings, and votes at every level of California government. Murder State is a comprehensive examination of these events and their early legacy. Preconceptions about Native Americans as shaped by the popular press and by immigrants’ experiences on the Overland Trail to California were used to further justify the elimination of Native people in the newcomers’ quest for land. The allegedly “violent nature” of Native people was often merely their reaction to the atrocities committed against them as they were driven from their ancestral lands and alienated from their traditional resources. In this narrative history employing numerous primary sources and the latest interdisciplinary scholarship on genocide, Brendan C. Lindsay examines the darker side of California history, one rarely studied in detail, and the motives of both Native Americans and Euro-Americans at the time. Murder State calls attention to the misuse of democracy to justify and commit genocide.