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RNA molecules participate in and regulate a vast array of cellular processes, and the scientific community is now entering a new era in which some aspect of RNA biology--as a tool, a therapeutic, a diagnostic, or part of a fundamental process--is becoming increasingly important. But initiating RNA research can be intimidating, and without a thorough understanding of the challenges and complexities inherent in handling this fragile nucleic acid, forays into the RNA world can be quite frustrating. RNA: A Laboratory Manual provides a broad range of up-to-date techniques so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the most sophisticated. Originating in four of the field's most prominent laboratories and written with novices as well as more advanced researchers in mind, this manual provides the necessary background and strategies for approaching any RNA investigation in addition to detailed step-by-step protocols and extensive tips and troubleshooting information. RNA: A Laboratory Manual will enable any researcher to approach a wide variety of RNA-related problems with confidence and a high expectation of success.

This publication summarizes the current status of our understanding of RNA, with particular emphasis on the chemistry of this key biological molecule. The various RNAs covered are messenger RNA, ribosomal RNA, transfer RNA and RNA enzymes (ribozymes). The different chapters detail biophysical and chemical methods to investigate RNA structure and function, the synthesis of native and modified RNAs and the latest advances in our understanding of the vast array of biological processes in which RNA is involved.

RNA (ribonucleic acid) molecules drive much of cellular action. Messenger RNA (mRNA) relays genetic information from DNA to ribosomes to direct the formation of proteins. At the same time, noncoding RNAs act as enzymes, scaffolds, and regulatory molecules, contributing greatly to the regulation of gene activity, RNA splicing, protein synthesis, and even DNA replication.This new edition of the highly successful RNA Worlds series focuses on emerging tools for analysis of RNA biology and how these tools are driving our understanding of RNA structure and function. The contributors review new approaches for imaging RNA molecules in cells, techniques for analyzing the behavior of single RNA molecules in vitro and in vivo, transcriptome-wide analyses, and novel methods for determining the structure of RNA and RNP (ribonucleoprotein) complexes. They discuss the insights these provide into the transcription and splicing machinery, ribosomes, reverse transcriptases, and subcellular structures such as stress granules.Also included are chapters exploring CRISPR editing, RNA switches for use in synthetic biology, and development of novel drugs targeting pathogenic RNA structures. In addition, the book covers recent insights into the primordial RNA World, including protocells and homochirality. It will therefore be of interest not only to biochemists and molecular biologists but to evolutionary biologists pondering the emergence of life on Earth.

RNA molecules participate in and regulate a vast array of cellular processes, and the scientific community is now entering a new era in which some aspect of RNA biology--as a tool, a therapeutic, a diagnostic, or part of a fundamental process--is becoming increasingly important. But initiating RNA research can be intimidating, and without a thorough understanding of the challenges and complexities inherent in handling this fragile nucleic acid, forays into the RNA world can be quite frustrating. RNA: A Laboratory Manual provides a broad range of up-to-date techniques so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the most sophisticated. Originating in four of the field's most prominent laboratories and written with novices as well as more advanced researchers in mind, this manual provides the necessary background and strategies for approaching any RNA investigation in addition to detailed step-by-step protocols and extensive tips and troubleshooting information. RNA: A Laboratory Manual will enable any researcher to approach a wide variety of RNA-related problems with confidence and a high expectation of success.

This book offers a balanced and integrated treatment of molecular biology, cell biology, and biochemistry. The central topics of molecular biology are included, including DNA structure, messenger RNA gene structure and activity, and the molecular methods for studying these genes.