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A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.

The renowned AIDS researcher Robert Gallo tells his story of scientific breakthrough in a riveting portrait of the people, the politics, and the pace of modern scientific discovery.

At an April 1984 press conference, government researchers announced that the cause of AIDS--the disease then terrifying the nation as if it were a Biblical scourge--was a "retrovirus" called HIV. Many scientists, including two Nobel winners, said it wasn't possible. But they were quickly drowned out by the ecstatic response from activists, government-funded researchers, a relieved public and, especially, the pharmaceutical industry, which quickly offered a treatment for HIV--a drug called called AZT. Within four years, the entire first group of AZT test subjects was dead. But the idea that HIV caused AIDS became so entrenched that international policy was being based on it, while big pharma raked in billions. Scientists who disagreed found themselves ostracized, their funding cut off. Journalist who raised questions were subject to vicious attacks from politicians and activists. Celia Farber has covered the tumultuous story in all its facets for over 20 years, including: disastrous National Institutes of Health drug tests on mothers and children in Africa, Tennessee and New York City; extensive interviews with blacklisted researchers and scientific dissidents such as Berkeley's Peter Duseberg and NIH renegade Jonathan Fishbein; and reporting from South Africa on the influence of pharmaceutical companies on foreign aid and policy. It is an astonishing and largely unknown story, and in Serious Adverse Events, Farber chronicles the entire history of AIDS, its triumphs and its failures, with astonishing research and mind-opening candor.

On July 22, 2009, a special meeting was held with twenty-four leading scientists at the National Institutes of Health to discuss early findings that a newly discovered retrovirus was linked to chronic fatigue syndrome (CFS), prostate cancer, lymphoma, and eventually neurodevelopmental disorders in children. When Dr. Judy Mikovits finished her presentation the room was silent for a moment, then one of the scientists said, Oh my God!” The resulting investigation would be like no other in science.For Dr. Mikovits, a twenty-year veteran of the National Cancer Institute, this was the midpoint of a five-year journey that would start with the founding of the Whittemore-Peterson Institute for Neuro-Immune Disease at the University of Nevada, Reno, and end with her as a witness for the federal government against her former employer, Harvey Whittemore, for illegal campaign contributions to Senate Majority Leader Harry Reid.On this journey Dr. Mikovits would face the scientific prejudices against CFS, wander into the minefield that is autism, and through it all struggle to maintain her faith in God and the profession to which she had dedicated her life. This is a story for anybody interested in the peril and promise of science at the very highest levels in our country.

Nick Fury has a dangerous mission for the Punisher: break into a Siberian nuclear missile silo and steal a lethal retrovirus. But Castle's mission soon sets off a chain reaction that will bring him into conflict with a ruthless Russian General - and take the world to the brink of war. Then, mobster Nicky Cavella returns to plague the Punisher, but when his scheme unleashes a wave of violence that rocks New York to its core, other predators start to emerge from the darkness.COLLECTING: PUNISHER (2004) 13-30

Nick Fury's got a job that needs doing - in Russia. The kind of suicide mission any sane man wouldn't ponder for a second. And that's exactly why he needs the Punisher. Searching for a deadly retrovirus whose sale on the black market could bring the world to its knees, Frank Castle penetrates a Russian nuclear silo...and comes face-to-face with the Mongolian! Collects Punisher #13-18.